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Index one: the i7 index column is within the forward orientation (match the orientation Should the i7 indexes while in the sample sheet).
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Refer to Index 2 (i5) Orientation For more info on how to enter i5 bases over the sample sheet in forward or reverse complement orientation.
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Set D Index Adapters Make reference to Index two (i5) Orientation For more info regarding how to enter i5 bases to the sample sheet in ahead or reverse enhance orientation.
Which means that these program work on the assumption that the i5 sequence is always inside the ahead orientation and will instantly generate the reverse enhance from the sequences provided for the suitable instrument type.
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